10X SDS-PAGE Running Gel Buffer
Quick Overview for 10X SDS-PAGE Running Gel Buffer (ABIN925557)
应用范围
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Supplier Product No.
- mb-017
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Supplier
- Rockland
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原理
- 10X SDS-PAGE Running Gel Buffer
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纯化方法
- 10X SDS-PAGE Running Buffer consists of 0.25 M Tris HCl, 1.92 M Glycine and 1% (w/v) Sodium Dodecyl Sulfate (SDS), pH 8.3. Meticulously prepared using ultra-pure reagents dissolved in highly polished pharmaceutical grade deionized water. Some yellow col
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过滤
- Sterile filtered
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Blocking Buffer for Fluorescent Western Blotting
CA, ELISA, IF, IHC, IP, MA, WB 1 X
10X TTBS Buffer pH 7.5ELISA, WB 10 X
Fluoromount-G®FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL
10X PBS Buffer pH 7.2ELISA, FACS, IF, WB 10 X
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应用备注
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Application Note: This product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water (or equivalent) to its final working concentration. No pH adjustment is required.
Western Blot Dilution: User Optimized
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 X
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注意事项
- Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature
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储存条件
- RT,4 °C
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储存方法
- Store container at room temperature (18° to 26° C) prior to opening. If desired, the solution may be stored at 4° C or less. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18° to 26° C) t Expiration date is six (6) months from date of receipt.
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有效期
- 6 months
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背景
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Synonyms: Sodium dodecyl sulfate polyacrylamide running buffer, Protein Gel Running Buffer, polyacrylamide gel running buffer
Background: SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no
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